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© Research
Publication : Scanning microscopy

Digital correlation of ion and optical microscopic images: application to the study of thyroglobulin chemical modification

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Scanning microscopy - 01 Dec 1990

Olivo JC, Kahn E, Halpern S, Fragu P

Link to Pubmed [PMID] – 2094006

Scanning Microsc. 1990 Dec;4(4):825-8

A method has been developed in order to digitally correlate ion and optical microscopic images of the same sample areas. Serial cross-sections of human thyroid tissue were analyzed by secondary ion mass microscopy and by light microscopy. The resulting chemical and immunochemical map images were superimposed and correlated by means of a two-pass registration algorithm which allows to correct for geometrical distortions introduced by the ion microscope. Results are presented for the study of thyroglobulin chemical modification in pathological thyroid tissue that demonstrates heterogeneous molecular activity.

http://www.ncbi.nlm.nih.gov/pubmed/2094006