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© Research
Publication : Virology

Dengue virus replicates and accumulates in Aedes aegypti salivary glands

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Virology - 18 Apr 2017

Raquin V, Lambrechts L

Link to Pubmed [PMID] – 28431281

Virology 2017 Apr;507:75-81

Dengue virus (DENV) is an RNA virus transmitted among humans by mosquito vectors, mainly Aedes aegypti. DENV transmission requires viral dissemination from the mosquito midgut to the salivary glands. During this process the virus undergoes several population bottlenecks, which are stochastic reductions in population size that restrict intra-host viral genetic diversity and limit the efficiency of natural selection. Despite the implications for virus transmission and evolution, DENV replication in salivary glands has not been directly demonstrated. Here, we used a strand-specific quantitative RT-PCR assay to demonstrate that negative-strand DENV RNA is produced in Ae. aegypti salivary glands, providing conclusive evidence that viral replication occurs in this tissue. Furthermore, we showed that the concentration of DENV genomic RNA in salivary glands increases significantly over time, indicating that active replication likely replenishes DENV genetic diversity prior to transmission. These findings improve our understanding of the biological determinants of DENV fitness and evolution.

https://www.ncbi.nlm.nih.gov/pubmed/28431281