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© A. Chauhan, JM Ghigo, C. Beloin.
Biofilm formation by Pseudomonas aeruginosa bacteria in Central Venous Catheter. Bacteria are stained in yellow and blood cells in red.
Publication : The Journal of Biological Chemistry

Contribution of DNA conformation and topology in right-handed DNA wrapping by the Bacillus subtilis LrpC protein

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The Journal of Biological Chemistry - 27 Nov 2002

Beloin C, Jeusset J, Revet B, Mirambeau G, Le Hégarat F, Le Cam E.

Link to Pubmed [PMID] – 12458218

J Biol Chem. 2003 Feb 14;278(7):5333-42. Epub 2002 Nov 27.

The Bacillus subtilis LrpC protein belongs to the Lrp/AsnC family of transcriptional regulators. It binds the upstream region of the lrpC gene and autoregulates its expression. In this study, we have dissected the mechanisms that govern the interaction of LrpC with DNA by electrophoretic mobility shift assay, electron microscopy, and atomic force microscopy. LrpC is a structure-specific DNA binding protein that forms stable complexes with curved sequences containing phased A tracts and wraps DNA to form spherical, nucleosome-like structures. Formation of such wraps, initiated by cooperative binding of LrpC to DNA, results from optimal protein/protein interactions specified by the DNA conformation. In addition, we have demonstrated that LrpC constrains positive supercoils by wrapping the DNA in a right-handed superhelix, as visualized by electron microscopy.