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© Pierre Gounon
Entrée de Listeria dans une cellule épithéliale (Grossissement X 10000). Image colorisée.
Publication : Molecular & general genetics : MGG

Construction and expression of a hybrid plasmid containing the Escherichia coli thrA and thrB genes

Team: Bacteria-Cell Interactions
Member: Pascale Cossart
Member: Moshe Yaniv
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molecular & general genetics : MGG - 01 Aug 1979

Cossart P, Katinka M, Yaniv M, Saint Girons I, Cohen GN

Link to Pubmed [PMID] – 390305

Mol. Gen. Genet. 1979 Aug;175(1):39-44

In vitro recombination techniques were used to clone the Escherichia coli thrA and thrB structural genes in the plasmid vector pBR322. The chimeric plasmid was analyzed and characterized genetically, by restriction mapping and DNA sequencing. The limited expression of the threonine biosynthetic enzymes in the strain carrying the recombinant plasmid is discussed.