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© Pierre Gounon
Entrée de Listeria dans une cellule épithéliale (Grossissement X 10000). Image colorisée.
Publication : Annales de microbiologie

[Clonage of the “malA” region of “Escherichia coli” K12: nucleotide sequence of the regulatory region and the promoters, identification and purification of the MalT-activator protein (author’s transl)]

Team: Bacteria-Cell Interactions
Team: Biology of Gram-Positive Pathogens
Member: Pascale Cossart
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Annales de microbiologie - 01 Jan 1982

Raibaud O, Débarbouillé M, Cossart P

Link to Pubmed [PMID] – 6462088

Ann. Microbiol. (Paris) 1982 Jan;133A(1):59-63

A 5,800-bp (base pair) HindIII-EcoRI DNA fragment containing malT, the positive regulator gene of the maltose regulon, and most of malP, the structural gene for maltodextrin phosphorylase, was cloned into pBR322. A sequence of 802 bp was established in a DNA segment containing the promotor for malPQ and the promoter for malT. A total of 611 bp separates the initiation codons for these two genes, which are transcribed in opposite directions. The malT product was identified as a 94,000 dalton polypeptide.