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© Research
Publication : Asian journal of organic chemistry

Benzoyl and pivaloyl as efficient protecting groups for controlled enzymatic synthesis of DNA and XNA oligonucleotides

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Asian journal of organic chemistry - 26 Aug 2022

Flamme, M.; Katkevica, D.; Pajuste, K.; Katkevics, M.; Sabat, N.; Hanlon, S.; Marzuoli, I.; Püntener, K.; Sladojevich, F.; Hollenstein, M.

Link to DOI – 10.1002/ajoc.202200384

Asian J. Org. Chem. 2022, e202200384

Controlled enzymatic synthesis is an alluring alternative to solid-phase synthesis and polymerase-mediated incorporation of nucleotides for the crafting of chemically modified, therapeutic oligonucleotides. While this approach has met some success for the elaboration of long, unmodified DNA sequences, very little research efforts have been dedicated to xeno nucleic acids (XNAs). Here, we have evaluated the possibility of using various 3’- O -blocking groups for controlled synthesis of locked nucleic acids (LNA). LNA nucleosides were equipped with protecting groups used in synthetic organic chemistry and were evaluated for their stability. The most promising candidates, benzoyl- and pivaloyl-protected nucleosides, were converted to the corresponding nucleotides. The resulting modified nucleotides were shown to be accepted by various polymerases. While single incorporation events were observed in high yields, strong esterase activity of polymerases represents a lasting hurdle that needs to be overcome. Overall, this article represents an additional step towards the controlled enzymatic synthesis of LNA-containing oligonucleotides and could be extended to other sugar or nucleobase modified nucleotides.

https://onlinelibrary.wiley.com/doi/10.1002/ajoc.202200384