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© Thomas Wollert
Publication : The FEBS journal

Autophagy in the test tube: In vitro reconstitution of aspects of autophagosome biogenesis

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The FEBS journal - 06 Feb 2016

Rao Y, Matscheko N, Wollert T

Link to Pubmed [PMID] – 26797728

FEBS J. 2016 06;283(11):2034-43

Autophagy is a versatile recycling pathway that delivers cytoplasmic contents to lysosomal compartments for degradation. It involves the formation of a cup-shaped membrane that expands to capture cargo. After the cargo has been entirely enclosed, the membrane is sealed to generate a double-membrane-enclosed compartment, termed the autophagosome. Depending on the physiological state of the cell, the cargo is selected either specifically or non-specifically. The process involves a highly conserved set of autophagy-related proteins. Reconstitution of their action on model membranes in vitro has contributed tremendously to our understanding of autophagosome biogenesis. This review will focus on various in vitro techniques that have been employed to decipher the function of the autophagic core machinery.

https://www.ncbi.nlm.nih.gov/pubmed/26797728