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© Therese Couderc, Marc Lecuit
Publication : Biologicals : journal of the International Association of Biological Standardization

Analysis by high throughput sequencing of Specific Pathogen Free eggs

Team: Biology of infection
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Biologicals : journal of the International Association of Biological Standardization - 13 Jun 2014

Gagnieur L, Cheval J, Cochet M, Breard E, Gratigny M, Hébert C, Muth E, Viarouge C, Dumarest M, Coulpier M, Eloit M

Link to Pubmed [PMID] – 24930452

Biologicals 2014 Jul;42(4):218-9

Specific Pathogen Free (SPF) embryonated eggs are used for the production of many veterinary and human vaccines. We have used High Throughput Sequencing to screen allantoic fluids and embryos for the presence of encapsidated viral genomes and viral transcripts, respectively. SPF eggs from two different producers were tested. We evidenced sequences corresponding to known endogenous retroviruses and sequences of Avian Leukosis Virus, but no sequence that might suggest a productive infection of eggs with a virus even distant from known viruses. Our results strongly suggest that SPF eggs such as those used for this study represent a safe substrate for the production of vaccines.