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© Pierre Gounon
Entrée de Listeria dans une cellule épithéliale (Grossissement X 10000). Image colorisée.
Publication : Cell

An RNA thermosensor controls expression of virulence genes in Listeria monocytogenes

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Cell - 01 Sep 2002

Johansson J, Mandin P, Renzoni A, Chiaruttini C, Springer M, Cossart P

Link to Pubmed [PMID] – 12230973

Cell 2002 Sep;110(5):551-61

In Listeria monocytogenes, virulence genes are maximally expressed at 37 degrees C, almost silent at 30 degrees C and controlled by PrfA, a transcriptional activator whose expression is thermoregulated. Here, we show that the untranslated mRNA (UTR) preceding prfA, forms a secondary structure, which masks the ribosome binding region. Mutations predicted to destabilize this structure led to virulence gene expression and invasion of mammalian cells at 30 degrees C. Chemical probing, native gel electrophoresis, in vitro translation, and “compensatory” and “increased stability” mutations demonstrated that the UTR switches between a structure active at high temperatures, and another inactive at low temperatures. Strikingly, when the DNA corresponding to the UTR was fused to gfp in E. coli, bacteria became fluorescent at 37 degrees C, but not at 30 degrees C. This mechanism of posttranscriptional thermoregulation may have important applications.

http://www.ncbi.nlm.nih.gov/pubmed/12230973