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© Research
Publication : Letters in applied microbiology

A sensitive and rapid method to determine the viability of freeze-dried bacterial cells

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Letters in applied microbiology - 01 Jan 2003

Parthuisot N, Catala P, Lebaron P, Clermont D, Bizet C

Link to Pubmed [PMID] – 12753251

Lett. Appl. Microbiol. 2003;36(6):412-7

AIMS: To determine the potential use of flow cytometry for viability asssessment of freeze-dried bacterial cells.

METHODS AND RESULTS: Escherichia coli CIP 54.8T and Vibrio metschnikovii CIP 104262 were analysed. The viability of freeze-dried cells resuspended in a nutrient broth was evaluated by culture whereas activity was determined by flow cytometry analysis of both esterase activity and cell death. Activity assessment by flow cytometry was found to be a rapid and good indicator of cell viability and was very efficient for quality control. For V. metschnikovii the fraction of active cells varies greatly depending on the freeze-drying procedure and within a given procedure.

CONCLUSIONS, SIGNIFICANCE AND IMPACT OF THE STUDY: Bacterial activity assessment by flow cytometry is very efficient for the control of freeze-dried cells.

http://www.ncbi.nlm.nih.gov/pubmed/12753251