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© Christophe Thomas, Institut Pasteur
Gel d’électrophorèse lors de la purification d’un échantillon protéique
Project

Implementation of automated procedures for high-throughput screening cultures and analytical purification of protein

Team: Production and Purification of Recombinant Proteins Technological Platform
Project leader: Stéphane Petres
Member: Mireille Nowakowski
Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
04
Feb 2015
Status
Ongoing
Members
2
Structures
1

About

The availability of recombinant proteins remains a bottleneck for many scientific projects. E.coli is the most widely expression system used, but the production of soluble proteins requires the simultaneous assessment and combination of multiple parameters. The PFPR is working on setting up HTP automated procedures on a Tecan platform to ensure the cloning of a large number of target genes, expression and analytical purification of proteins, through a standardized pipeline.