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© Institut Pasteur
Spirochète : bactérie hélicoïdale, flexible et ondulante de longueur variable, non colorable par la coloration de Gram, très mobile (endoflagelles). Trois familles : Spirochaetaceae, Leptospiraceae, et Brachyspiraceae. Principaux genres pathogènes pour l'homme : Borrelia (Borrelia burgdorferi cause de la maladie de Lyme), Treponema (Treponema pallidum cause de la syphillis), Leptospira (Leptospira interrogans serovar icterohaemorrhagiae cause de la maladie de Weil). Image colorisée.
Project

Cell morphology and motility in Leptospira spp.

Scientific Fields
Diseases
Organisms
Applications
Technique
Starting Date
15
Aug 2015
Status
Ongoing
Members
1
Structures
1

About

Leptospira spp. are thin, highly motile, slow-growing spirochetes that can be distinguished from other bacteria on the basis of their unique helical shape and the presence of periplasmic flagella.

morpholeptoWe showed that peptidoglycan sacculi from Leptospira spp. retain the helical shape of intact cells. Interestingly, the distribution of muropeptides was different from that in the E. coli model, indicating that specific enzymes might be active on the peptidoglycan macromolecule. We could alter the shape of L. biflexa with the broad-spectrum β-lactam antibiotics that preferentially target penicillin-binding proteins. Loss of the MreB function was correlated with morphological abnormalities such as a localized increased diameter and heterogeneous length. These findings indicate that important aspects of leptospiral cell morphology are determined by the cytoskeleton and the murein layer, thus providing a starting point for a better understanding of the morphogenesis in these atypical bacteria .

 

mouvemtlepto1.jpg

 

Movement of leptospires (A, from Goldstein and Charon, 1988) and electronic microscopy of Leptospira biflexa (B). During translational motility, counterclockwise rotation of the perisplasmic flagellum produces spiral-shaped ends at the leading edge while concomitantly, clockwise PF rotation causes gyrating cell ends at the tail to remain hooked-shaped

 

The basal body and hook structures of spirochete flagella are similar to those of the model systems of Escherichia coli and Salmonella enterica. However, in contrast to the flagellin polymer of enterobacteria, the flagellar filaments of spirochetes investigated to date have a complex structure comprising FlaA and FlaB proteins. In Leptospira spp. the flaA genes are organized in a locus with flaA2 immediately upstream of flaA1. We showed that the flaA1 and flaA2 transposon mutants were able to assemble periplasmic flagella, but their motility, virulence, and flagellar and cellular morphologies were affected, depending on whether FlaA1 or both FlaA proteins were expressed. Our most recent studies using whole genome sequencing of spontaneous non-motile strains and preliminary screening of a library of transposon mutants for motility-deficient mutants allowed the identification of two novel flagellar proteins named FcpA and FcpB