Interplay between influenza virus and the ubiquitin/proteasome system
As a major pathway of reversible protein modification in eukaryotes cells, leading to their degradation, affecting their subcellular localization, or activity, the Ubiquitin Proteasome System (UPS) is a prime target of viruses. Recent evidence […]
Multi-species mapping of influenza viruses cellular targets using high-throughput complementary strategies in a “One Health” perspective.
Despite medical progress, the recent outbreaks of viral zoonoses (e.g. MERS-CoV, Ebola virus disease) illustrate the constant threat represented by viruses in a world in motion and globalization. These epidemic events stress the need […]
Comparative interactomics of filoviridae (Task Force Ebola WP6.2)
To date, knowledge on the interaction network between filovirus proteins and the host proteome (virhostome) are still extremely small and fragmented. These studies are limited to a few proteins such as VP24, VP35 and […]
Optogenetic “Cre-stiching” strategy for barcode fusion genetics
we developed an optogenetic “Cre-stiching” strategy for barcode fusion genetics. This new technology is based on light-inducible Cre-recombinase complementation. The resulting optogenetic control Cre-recombinase produces a concatenated DNA, linking together genetic barcodes. These concatenated […]
High-throughput protein-protein interaction assay based on a split-luciferase
The coverage and robustness of protein-protein interactions (PPI) datasets can be markedly increased by combining orthogonal PPI assays. For this purpose we have recently developed two variations of our High-throughput Gaussia princeps Complementation Assay(HT-GPCA), […]
2018The SRC-family tyrosine kinase HCK shapes the landscape of SKAP2 interactome, Oncotarget 2018 Mar;9(17):13102-13115.
2017Inhibition of the inflammatory response to stress by targeting interaction between PKR and its cellular activator PACT, Sci Rep. 2017 Nov 23;7(1):16129.
2015Quantifying domain-ligand affinities and specificities by high-throughput holdup assay, Nat. Methods 2015 Aug;12(8):787-93.
2013A comparative approach to characterize the landscape of host-pathogen protein-protein interactions, J Vis Exp 2013;(77):e50404.
2012Comparative analysis of virus-host interactomes with a mammalian high-throughput protein complementation assay based on Gaussia princeps luciferase, Methods 2012 Dec;58(4):349-59.
2012Mapping of Chikungunya virus interactions with host proteins identified nsP2 as a highly connected viral component., J Virol 2012 Mar; 86(6): 3121-34.
2011Benchmarking a luciferase complementation assay for detecting protein complexes, Nat. Methods 2011 Dec;8(12):990-2.
2009ViralORFeome: an integrated database to generate a versatile collection of viral ORFs, Nucleic Acids Res. 2010 Jan;38(Database issue):D371-8.
2009The EVER proteins as a natural barrier against papillomaviruses: a new insight into the pathogenesis of human papillomavirus infections, Microbiol. Mol. Biol. Rev. 2009 Jun;73(2):348-70.
2007Regulation of cellular zinc balance as a potential mechanism of EVER-mediated protection against pathogenesis by cutaneous oncogenic human papillomaviruses, J. Exp. Med. 2008 Jan;205(1):35-42.
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