D. Bikard’s team is studying the genetic systems that are emerging as a result of the arms race between bacteria and bacteriophages, and how these systems can be exploited as new biotechnological tools. In particular, the group is interested in the catalytically dead Cas9 protein variant (dCas9) and its ability to control gene expression, a mechanism also known as CRISPRi. My role to date has been to develop protocols for constructing DNA libraries from CRISPRi experiments, using drop PCR methods in particular.
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2024A virally-encoded tRNA neutralizes the PARIS antiviral defence system., Nature 2024 Aug; (): .
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2023Functional maturation of human iPSC-derived pyramidal neurons in vivo is dependent on proximity with the host tissue., Front Cell Neurosci 2023 ; 17(): 1259712.
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2020High-throughput single-cell activity-based screening and sequencing of antibodies using droplet microfluidics, Nat Biotechnol . 2020 Jun;38(6):715-721. doi: 10.1038/s41587-020-0466-7. Epub 2020 Mar 30..