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About

Originally from Pamplona (Spain), where I studied biology & biochemistry, but I have been living in Tromsø (Norway) for my Erasmus year, in Toronto (Canada) for master’s project, and in Frankfurt am Main (Germany) for my PhD.

Now here, in Paris, I am a postdoctoral researcher interested in neuroscience, immunology, the brain barriers, and high-throughput technologies to study brain-immune communications, ageing, circadian rhythm, and diseases such as Alzheimer’s.

CV

amaia.dominguez-belloso@pasteur.fr

Place and date of birth : Pamplona, Navarra, Spain 27.03.1990

 

Latest position

2016-2020 PhD thesis, team of PD Dr. rer. nat. Stefan Liebner (Goethe University Frankfurt). Thesis: The role of the blood-brain barrier in Alzheimer’s disease

 

Research skills

  • Animal handling in vivo: behavioural testing for mice, IP&IV injections, full-body perfusions
  • Animal handling post-mortem: mouse brain microvessel isolation, tissue clearing, dissections, slice preparations, RNA&DNA isolation
  • Bioinformatics: analysis of 3’ RNA-Seq data, R & R Studio, Prism, NIS, Imaris, Affinity & Photo Designer, Nikon-specific software, excel
  • Microscopy: confocal, live cell, fluorescence and light-sheet fluorescence
  • In vitro cell lines: bEnd5, MBE, HEK293T; stable gene knock-down generation by lentiviral infection, assessment of migration and proliferation properties
  • Biochemistry: qPCRs, WB, stainings, luciferase assays

 

Teaching experience

Supervision of master student’s work in the lab : 12 weeks

Supervision of bachelor student’s work in the lab :12 weeks

 

Educational background

2013-2015 Master in Neuroscience. Autonomous University of Madrid (Spain).

2014-2015 Thesis work at York University (Toronto, Canada). Title: Designing and Expression of Different Panx1 Mutants and Fusion Proteins, and an attempt of characterization

2008-2013 Double degree in Biology and Biochemistry. University of Navarre (Spain).

2012-2013 ERASMUS Exchange Study Program at the University of Tromsø (Norway). Bachelor’s thesis in biology: Purification and Characterization of Porcine Brain eNPP6

 

Relevant workshops and courses 

Neuro -anatomy & -pathology workshop : Jan. 2018, VU Amsterdam

Biostatistics workshop : Jan. 2018, VU Amsterdam

Next generation sequencing workshop : June 2017, GenXPro

Brain barriers in vitro training : June 2017, Dr. M. Culot & Dr. M. Deli

Scitec-Media : Mar. 2017, Beat Glogger

3D-imaging workshop : Mar. 2017, LaVision

Zebra fish workshop : Mar. 2017, Prof. Dr. B. Vanhollebeke

Bioinformatics workshop : Mar. 2016, University of Bern

Training Course in Directing and Designing procedures for Experimental Animals : Jan.-Feb. 2014, UAM

 

Grants and awards

Best poster presentation by Fluids and Barriers of the CNS at the Barriers of the CNS, Gordon Research Conference, June 17th-22nd, 2018, Colby-Sawyer College, NH, USA
https://fluidsbarrierscns.biomedcentral.com/

Marie Skłodowska Curie Fellowship (PhD position) H2020-MSCA-ITN-2015 675619, ESR 9. https://www.btrain-2020.eu/esrNine

Convenios Internacionales Scholarship to conduct my master’s project abroad, by the Autonomous University of Madrid, Sep. 2014

Erasmus Program Scholarship to study a full academic year abroad, Aug. 2012

 

Publications

Preview: Dominguez-Belloso A., & Liebner, S. (2018). The Fountain of Youth: It’s All in Our Veins. Neuron, 100(1), 9–11. http://doi.org/10.1016/j.neuron.2018.09.038

In preparation: Dominguez-Belloso A., et al. Transcriptomic profiling and in vivo characterization of the BBB in AD reveal new therapeutic targets