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© Pierre Lafaye
Astrocytes marqués par des anticorps VHH anti-GFAP. Des anticorps d'alpagas dirigés contre une protéine spécifique des astrocytes, la GFAP (Glial Fibrillary Acidic Protein), ont été obtenus à partir de camélidés immunisés. La partie VHH (partie de l'anticorps qui reconnaît l'antigène) a été exprimée sous forme recombinante chez Escherichia coli.
Scientific Fields
Diseases
Organisms
Applications
Technique
Application Deadline
31
Dec 2017
2017-12-31 8:00:00 2017-12-31 18:00:00 Europe/Paris High-throughput screening for VHH/nanobodies discovery Missions We are seeking for a highly motivated research scientist with experience in antibody discovery to join a project developed by 3 labs, the Antibody engineering platform, the Unit of Antibodies in Therapy and […] "25-28 rue du Dr. Roux, 75015, Paris" Pierre Lafaye plafaye@pasteur.fr

Missions

We are seeking for a highly motivated research scientist with experience in antibody discovery to join a project developed by 3 labs, the Antibody engineering platform, the Unit of Antibodies in Therapy and Pathology (both Institut Pasteur, Paris) and the Immunovirology lab (Institut Pasteur, Montevideo). The candidate will work at the Antibody engineering platform in collaboration with the 2 other labs.

The project global aim is to help developing a pipeline for high-throughput discovery of alpaca VHH nanobodies. This pipeline includes alpaca immunization, B cell differentiation in vitro, droplet microfluidic sorting of B cells of interest, VH sequencing by NGS, antibody cloning and expression, antibody validation.

The candidate will be entrusted with B cell differentiation in vitro, VH sequencing by NGS, antibody cloning and expression, antibody validation. He/she will learn droplet microfluidic sorting of B cells of interest in collaboration with a microfluidicist from the consortium.

Novel assays and methods of analysis will have to be developed, including plasmocyte differentiation from alpaca memory B cells, repertoire analysis of VHH and antibody engineering.

The ideal candidate has expertise in molecular biology, B cell biology, and antibody biochemistry.

 

Profil

PhD in Protein/Antibody engineering and/or single cell technologies

Expertise in antibody engineering, high-throughput screening, antibody characterization is required.

A hands-on expertise in bioinformatics and repertoire analysis would be a plus.

An experience in cell culture will also be appreciated.

Capacity to work with the different partners is essential.

ABOUT THE POSITION
Job Title: Temporary employee
Location: Paris

Starting Date: January 2018- December 2019
Duration: 24 months.