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© Institut Pasteur / Alban Orsini
Le bâtiment Nocard qui abrite le microscope Titan KriosTM, à l'Institut Pasteur (Paris)
Scientific Fields
Diseases
Organisms
Applications
Technique
Application Deadline
30
Sep 2019
2019-09-30 8:00:00 2019-09-30 18:00:00 Europe/Paris Cryo-CLEM and in situ cellular cryo electron tomography specialist We are seeking a highly motivated individual to join our Structural Biology team as specialist to lead and support our cryo-CLEM and in situ cellular tomography efforts. Responsibilities • Maintaining eukaryotic cell culture and […] "25-28 rue du Dr. Roux, 75015, Paris" Dorit Hanein dorit.hanein@pasteur.fr

We are seeking a highly motivated individual to join our Structural Biology team as specialist to lead and support our cryo-CLEM and in situ cellular tomography efforts.

Responsibilities

• Maintaining eukaryotic cell culture and mastering sample preparation procedures for electron and light microscopy;
• Overseeing and providing guidance in all aspects of cell-culture sample preparation for light and electron microscopy to lab personnel and collaborators;
• Maintaining cell line stocks, various reagent collections and inventories for the unit;
• Training and supervision of new lab personnel;
• Preparing general lab reagents as pertaining to cell culture and imagining needs, ordering and stocking of lab supplies.

The successful applicant will work in a stimulating academic and cultural environment, and will have the opportunity to contribute as an author to manuscripts. The unit is equipped with cell culture, molecular biology, light and cryo-CLEM technology, as well as having access to the newly established Nanoimaging Core at Institut Pasteur, headed by Matthijn Vos. The state-of the-art core houses a 300kV Titan Krios equipped with a K3 direct electron detector and energy filter for high resolution structural studies of biological molecules in situ, 2 screening 200 kV Glacios electron microscopes, as well as one Aquilos and all required ancillary equipment for cellular cryo-ET sample preparation.

Required Experience
• Strong hands-on expertise in microscopy, immuno-staining techniques, specimen preparation for light and electron microscopy, and sample evaluation;
• Strong hands-on expertise in molecular biology, protein biochemistry and antibody assays;
• Strong hands-on expertise in tissue culture;
• Hands-on experience in operation of electron cryo TEMs a plus;
• Bachelor’s degree in biological science, chemistry, physics or a related field, or an equivalent combination of education, training and experience from which comparable knowledge, skills and abilities have been attained;
• Requires a minimum of 7+ years of directly related experience to eukaryotic cell culture protocols and sample preparation procedures for electron and light microscopy;
• For candidates with a master’s degree, a minimum of 5+ years is required.

Required Skills
• The ability to independently characterize and troubleshoot eukaryotic cell sample preparations for light and fluorescence microscopy;
• The ability to rapidly learn new research assays and process the obtained data;
• The ability to work independently as well as in a team environment;
• The ability to manage multiple projects in a rapidly changing, deadline-driven environment;
• Strong analytical, time management, organizational and interpersonal skills;
• Excellent verbal/written English communication and skills.

Job Location
Institut Pasteur, Paris, France

Position Type
Permanent

Ready to join a team that is always innovating because we believe anything is possible? Our research team develops, employs and combines electron cryo-microscopy and cellular tomography with biophysical and computational approaches to allow direct visualization and quantification of macromolecular assemblies in 3D while in their native cellular environment. To join the Structural Studies of Macromolecular Machines in cellula Unit (Pr. Hanein) apply at: recrutement@pasteur.fr