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© Melanie Blokesch, EPFL
Flagellated Vibrio cholerae
Event

! CANCELLED ! “Revisiting DNA transfer by bacterial conjugation at the cellular scale” – GENOMES and GENETICS DEPARTMENT SEMINAR

Scientific Fields
Diseases
Organisms
Applications
Technique
Date
16
Apr 2020
Time
11:00:00
25-28 Rue du Dr Roux, 75015 Paris, France
Address
Building: rdc Centre François Jacob Room: auditorium du CFJ
Location
2020-04-16 11:00:00 2020-04-16 12:30:00 Europe/Paris ! CANCELLED ! “Revisiting DNA transfer by bacterial conjugation at the cellular scale” – GENOMES and GENETICS DEPARTMENT SEMINAR Abstract: Drug resistance dissemination by horizontal gene transfer remains poorly understood at the cellular scale. Using live-cell microscopy, we reveal the dynamics of resistance acquisition by transfer of the F conjugative plasmid encoding the tetracycline-efflux […] 25-28 Rue du Dr Roux, 75015 Paris, France Zeynep Baharoglu zeynep.baharoglu@pasteur.fr

About

Abstract:
Drug resistance dissemination by horizontal gene transfer remains poorly understood at the cellular scale. Using live-cell microscopy, we reveal the dynamics of resistance acquisition by transfer of the F conjugative plasmid encoding the tetracycline-efflux pump TetA. We show that the entry of the ssDNA plasmid into the recipient cell is rapidly followed by complementary strand synthesis and expression of newly acquired genes. TetA production is enhanced by zygotic induction resulting in the optimized establishment of resistance. In the presence of protein synthesis-inhibiting antibiotics, acquisition of resistance becomes strictly dependent on AcrAB-TolC multidrug efflux pump. We showed that AcrAB-TolC is required for maintenance of protein synthesis activity and therefore TetA production following plasmid acquisition. This work uncovers a novel essential role of multidrug efflux systems in the acquisition of drug-specific resistance by horizontal gene transfer and helps to understand the dissemination of antibiotic resistance in bacteria.

Partners

Location

Building: rdc Centre François Jacob
Room: auditorium du CFJ
Address: 25-28 Rue du Dr Roux, 75015 Paris, France