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© Research
Publication : Microbial genomics

LiSEQ – whole-genome sequencing of a cross-sectional survey of Listeria monocytogenes in ready-to-eat foods and human clinical cases in Europe.

Team: Biodiversity and Epidemiology of Bacterial Pathogens
Team: Biology of infection
Member: Sylvain Brisse
Member: Marc Lecuit
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Microbial genomics - 01 Feb 2019

Painset A, Björkman JT, Kiil K, Guillier L, Mariet JF, Félix B, Amar C, Rotariu O, Roussel S, Perez-Reche F, Brisse S, Moura A, Lecuit M, Forbes K, Strachan N, Grant K, Møller-Nielsen E, Dallman TJ,

Link to Pubmed [PMID] – 30775964

Link to DOI – e00025710.1099/mgen.0.000257

Microb Genom 2019 Feb; 5(2):

We present the LiSEQ (Listeria SEQuencing) project, funded by the European Food Safety Agency (EFSA) to compare Listeria monocytogenes isolates collected in the European Union from ready-to-eat foods, compartments along the food chain (e.g. food-producing animals, food-processing environments) and humans. In this article, we report the molecular characterization of a selection of this data set employing whole-genome sequencing analysis. We present an overview of the strain diversity observed in different sampled sources, and characterize the isolates based on their virulence and resistance profile. We integrate into our analysis the global L. monocytogenes genome collection described by Moura and colleagues in 2016 to assess the representativeness of the LiSEQ collection in the context of known L. monocytogenes strain diversity.