About
The microinjection of the CRISPR/Cas9 system in one-cell embryos is actually revolutionizing the in vivo genome-engineering field because of its big efficiency for KO/ KI (when a donor DNA matrix co-injected with the CRISPR/CAS9 system) generation. The system CRISPR/Cas9 is developing very rapidly due to its design simplicity and great versatility (possibility of multiple mutations in a single step modification) together with a great potential in increasing the levels of efficiency of targeted alterations.