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© Institut Pasteur
Spirochète : bactérie hélicoïdale, flexible et ondulante de longueur variable, non colorable par la coloration de Gram, très mobile (endoflagelles). Trois familles : Spirochaetaceae, Leptospiraceae, et Brachyspiraceae. Principaux genres pathogènes pour l'homme : Borrelia (Borrelia burgdorferi cause de la maladie de Lyme), Treponema (Treponema pallidum cause de la syphillis), Leptospira (Leptospira interrogans serovar icterohaemorrhagiae cause de la maladie de Weil). Image colorisée.
Publication : Journal of medical microbiology

A multilocus variable number tandem repeat analysis assay provides high discrimination for genotyping Leptospira santarosai strains

Team: Biology of Spirochetes
Reference Center: Leptospirosis
Member: Mathieu Picardeau
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of medical microbiology - 26 Feb 2015

Hamond C, Pinna M, Medeiros MA, Bourhy P, Lilenbaum W, Picardeau M

Link to Pubmed [PMID] – 25721051

J. Med. Microbiol. 2015 May;64(Pt 5):507-12

Considering the prevalence of Leptospira santarosai infections in the Americas and the scarce information about the species, we aimed to apply a multilocus variable number tandem repeat (VNTR) analysis (MLVA) for the molecular typing of L. santarosai isolates from various sources. Amplification of three VNTR loci selected from L. santarosai genome sequences resulted in a wide range of sizes for the amplified products amongst the 21 L. santarosai strains analysed. This suggested a variation in tandem repeat copy numbers in the VNTR loci. secY sequencing also showed a high nucleotide diversity, confirming the MLVA data. In conclusion, this novel MLVA provided a high level of discrimination between L. santarosai isolates, and this new typing tool could be used to investigate leptospirosis in regions where L. santarosai predominates.