Timeline

Hiroshi Sakamoto

Permanent Researcher

28 Jul 2015

project

Cyanobacterial bioactive compounds

28 Jul 2015

team

Collection of Cyanobacteria

11 May 2015

team

Biology of Host-parasite Interactions

29 Jun 2014

publication

Exonuclease-mediated degradation of nascent RNA silences genes linked to severe malaria

19 Dec 2011

publication

Clag9 is not essential for PfEMP1 surface expression in non-cytoadherent Plasmodium falciparum parasites with a chromosome 9 deletion

13 Dec 2011

publication

PfAlbas constitute a new eukaryotic DNA/RNA-binding protein family in malaria parasites

10 Nov 2005

publication

Towards systematic identification of Plasmodium essential genes by transposon shuttle mutagenesis

01 Oct 2004

publication

Conditional mutagenesis using site-specific recombination in Plasmodium berghei

01 Jul 2004

publication

Structure-function relationships of UMP kinases from pyrH mutants of Gram-negative bacteria

01 Feb 2004

publication

Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy

01 Jan 2004

publication

Identification and immunochemical location of UMP kinase from Bacillus subtilis

31 May 2002

publication

Comparative modelling and immunochemical reactivity of Escherichia coli UMP kinase

20 Nov 2001

publication

Solution structure and backbone dynamics of the defunct domain of calcium vector protein

01 Apr 2001

publication

Folding units in calcium vector protein of amphioxus: Structural and functional properties of its amino- and carboxy-terminal halves

01 Apr 2001

publication

Mass spectrometric analysis of recombinant adenylate cyclase toxin from Bordetella pertussis strain 18323/pHSP9

11 Jul 2000

publication

Sequential calcium binding to the regulatory domain of calcium vector protein reveals functional asymmetry and a novel mode of structural rearrangement

01 Jul 1999

publication

Assay of nucleoside 2-deoxyribosyltransferase activity with pyruvate kinase/lactate dehydrogenase coupling system

01 Feb 1999

publication

Immunochemical analysis of UMP kinase from Escherichia coli

15 Dec 1998

publication

Structures of escherichia coli CMP kinase alone and in complex with CDP: a new fold of the nucleoside monophosphate binding domain and insights into cytosine nucleotide specificity

01 Nov 1998

publication

1H and 15N resonance assignment of the calcium-bound form of the Nereis diversicolor sarcoplasmic Ca(2+)-binding protein

01 Aug 1998

publication

Substitution of an alanine residue for glycine 146 in TMP kinase from Escherichia coli is responsible for bacterial hypersensitivity to bromodeoxyuridine

01 Apr 1997

publication

Structural and catalytic properties of CMP kinase from Bacillus subtilis: a comparative analysis with the homologous enzyme from Escherichia coli

01 Feb 1996

publication

CMP kinase from Escherichia coli is structurally related to other nucleoside monophosphate kinases

18 Apr 1995

publication

Escherichia coli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine nucleotides and UTP

10 Feb 1995

publication

Enzymatic synthesis of guanine nucleotides labeled with 15N at the 2-amino group of the purine ring

01 Jul 1992

publication

Bordetella pertussis adenylate cyclase toxin. Structural and functional independence of the catalytic and hemolytic activities

01 Mar 1992

publication

Both adenylate cyclase and hemolytic activities are required by Bordetella pertussis to initiate infection

31 Jul 1991

publication

High-level synthesis of active adenylate cyclase toxin of Bordetella pertussis in a reconstructed Escherichia coli system

01 Oct 1990

publication

Deletions affecting hemolytic and toxin activities of Bordetella pertussis adenylate cyclase

01 Aug 1990

publication

Gene heterogeneity for tetracycline resistance in Staphylococcus spp

01 May 1990

publication

Synthesis and secretion of Bordetella pertussis adenylate cyclase as a 200-kilodalton protein

01 Jun 1989

publication

Comparison of enzyme-linked immunosorbent assay (ELISA) with dot hybridization using 32P- or 2-acetylaminofluorene (AAF)-labelled cDNA probes for the detection and characterization of beet necrotic yellow vein virus

01 Mar 1989

publication

Calibration of target amounts of DNA in hybridization experiments using monoclonal anti-nucleoside antibodies

01 Dec 1988

publication

Secretion of cyclolysin, the calmodulin-sensitive adenylate cyclase-haemolysin bifunctional protein of Bordetella pertussis

01 Dec 1988

publication

Non-radioactive gene probes for the detection of tetracycline and/or minocycline resistance in staphylococci

01 Mar 1987

publication

5-Bromodeoxyuridine in vivo labelling of M13 DNA, and its use as a non-radioactive probe for hybridization experiments

01 Jul 1984

publication

A little guide for advanced search:

Timeline

Cyanobacterial bioactive compounds

Collection of Cyanobacteria

Biology of Host-parasite Interactions

Exonuclease-mediated degradation of nascent RNA silences genes linked to severe malaria

Clag9 is not essential for PfEMP1 surface expression in non-cytoadherent Plasmodium falciparum parasites with a chromosome 9 deletion

PfAlbas constitute a new eukaryotic DNA/RNA-binding protein family in malaria parasites

Towards systematic identification of Plasmodium essential genes by transposon shuttle mutagenesis

Conditional mutagenesis using site-specific recombination in Plasmodium berghei

Structure-function relationships of UMP kinases from pyrH mutants of Gram-negative bacteria

Insight into the activation mechanism of Bordetella pertussis adenylate cyclase by calmodulin using fluorescence spectroscopy

Identification and immunochemical location of UMP kinase from Bacillus subtilis

Comparative modelling and immunochemical reactivity of Escherichia coli UMP kinase

Solution structure and backbone dynamics of the defunct domain of calcium vector protein

Folding units in calcium vector protein of amphioxus: Structural and functional properties of its amino- and carboxy-terminal halves

Mass spectrometric analysis of recombinant adenylate cyclase toxin from Bordetella pertussis strain 18323/pHSP9

Sequential calcium binding to the regulatory domain of calcium vector protein reveals functional asymmetry and a novel mode of structural rearrangement

Assay of nucleoside 2-deoxyribosyltransferase activity with pyruvate kinase/lactate dehydrogenase coupling system

Immunochemical analysis of UMP kinase from Escherichia coli

Structures of escherichia coli CMP kinase alone and in complex with CDP: a new fold of the nucleoside monophosphate binding domain and insights into cytosine nucleotide specificity

1H and 15N resonance assignment of the calcium-bound form of the Nereis diversicolor sarcoplasmic Ca(2+)-binding protein

Substitution of an alanine residue for glycine 146 in TMP kinase from Escherichia coli is responsible for bacterial hypersensitivity to bromodeoxyuridine

Structural and catalytic properties of CMP kinase from Bacillus subtilis: a comparative analysis with the homologous enzyme from Escherichia coli

CMP kinase from Escherichia coli is structurally related to other nucleoside monophosphate kinases

Escherichia coli UMP-kinase, a member of the aspartokinase family, is a hexamer regulated by guanine nucleotides and UTP

Enzymatic synthesis of guanine nucleotides labeled with 15N at the 2-amino group of the purine ring

Bordetella pertussis adenylate cyclase toxin. Structural and functional independence of the catalytic and hemolytic activities

Both adenylate cyclase and hemolytic activities are required by Bordetella pertussis to initiate infection

High-level synthesis of active adenylate cyclase toxin of Bordetella pertussis in a reconstructed Escherichia coli system

Deletions affecting hemolytic and toxin activities of Bordetella pertussis adenylate cyclase

Gene heterogeneity for tetracycline resistance in Staphylococcus spp

Synthesis and secretion of Bordetella pertussis adenylate cyclase as a 200-kilodalton protein

Comparison of enzyme-linked immunosorbent assay (ELISA) with dot hybridization using 32P- or 2-acetylaminofluorene (AAF)-labelled cDNA probes for the detection and characterization of beet necrotic yellow vein virus

Calibration of target amounts of DNA in hybridization experiments using monoclonal anti-nucleoside antibodies

Secretion of cyclolysin, the calmodulin-sensitive adenylate cyclase-haemolysin bifunctional protein of Bordetella pertussis

Non-radioactive gene probes for the detection of tetracycline and/or minocycline resistance in staphylococci

5-Bromodeoxyuridine in vivo labelling of M13 DNA, and its use as a non-radioactive probe for hybridization experiments

Cloning of the iron superoxide dismutase gene (sodB) in Escherichia coli K-12