Flow Cytometry protocols – handbook

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Immunology at work – resource center

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Handbook PDF => flow-cytometry-protocols-handbook

• • Sample preparation
    Isolation of human PBMCs from whole blood
    Mouse bone marrow cell isolation
    Mouse spleen cell isolation protocol
    Whole blood staining protocol for flow cytometry analysis Red blood cell lysis
  • Immune cell stimulation
    In vitro differentiation of macrophages from monocytes Stimulation of cytokine production in immune cells
    T cell activation
  • Phenotypic analysis
    Staining cell surface targets for flow cytometry
    Staining intracellular antigens for flow cytometry Intracellular staining guide for human and mouse cytokines Compensation
    Panel design
    Blocking non-specific binding with CellBlox™ Blocking Buffer
  • Cell viability and apoptosis
    Cell viability
    Annexin V staining for apoptosis detection Camptothecin induced apoptosis protocol
  • Cell cycle
    DNA content analysis in living cells
    DNA content in fixed cells
  • Cell proliferation
    Cell proliferation assay
    Generational analysis of T cell proliferation