Relief of catabolite repression in a cAMP-independent catabolite gene activator mutant of Escherichia coli

Lien vers Pubmed [PMID] – 14990258

Res. Microbiol. 2004 Mar;155(2):76-9

We isolated and characterized a new catabolite gene activator mutant (crp*) of Escherichia coli that confers cAMP-independent expression and total relief of catabolite repression of beta-galactosidase and tryptophanase synthesis. The two mutations responsible for this phenotype change the amino acids at codon 72 from Glu to Ala and at codon 144 from Ala to Thr in the corresponding CAP* protein.