Quantitative gene profiling of long noncoding RNAs with targeted RNA sequencing

Lien vers Pubmed [PMID] – 25751143

Nat. Methods 2015 Apr;12(4):339-42

We compared quantitative RT-PCR (qRT-PCR), RNA-seq and capture sequencing (CaptureSeq) in terms of their ability to assemble and quantify long noncoding RNAs and novel coding exons across 20 human tissues. CaptureSeq was superior for the detection and quantification of genes with low expression, showed little technical variation and accurately measured differential expression. This approach expands and refines previous annotations and simultaneously generates an expression atlas.