Paenibacillus auburnensis sp. nov. and Paenibacillus pseudetheri sp. nov., isolated from the rhizosphere of Zea mays

Lien vers Pubmed [PMID] – 37014794

Lien DOI – 10.1099/ijsem.0.005808

Int J Syst Evol Microbiol 2023 Apr; 73(4):

Two Gram-stain-positive, aerobic, endospore-forming bacterial strains, isolated from the rhizosphere of Zea mays were studied for their detailed taxonomic allocation. Based on 16S rRNA gene sequence similarity comparisons, both strains JJ-7^T and JJ-60^T were shown to be members of the genus Paenibacillus. Strain JJ-7^T was most closely related to the type strains of Paenibacillus tianjinensis (99.6%) and P. typhae (98.7%), and strain JJ-60^T to Paenibacillus etheri (99.5%). The 16S rRNA gene sequence similarities to all other Paenibacillus species were ≤98.4%. Both strains JJ-7^T and JJ-60^T showed 97.6% 16S rRNA gene sequence similarity between each other. Genomic comparisons showed that the average nucleotide identity and digital DNA-DNA hybridization values to next related type strain genomes were always <94 and <56%, respectively. The polar lipid profiles of both strains contain a number of phospholipids including diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine, which is in accord with the genus Paenibacillus. The major quinone was MK-7 in both strains. Major fatty acids were iso- and anteiso-branched. Physiological and biochemical characteristics allowed a further phenotypic differentiation of strains JJ-7^T and JJ-60^T from the most closely related species. Thus, each strain represents a novel species of the genus Paenibacillus, for which the names Paenibacillus auburnensis sp. nov. and Paenibacillus pseudetheri sp. nov. are proposed, with JJ-7^T (= CIP 111892^T = DSM 111785^T = LMG 32088^T = CCM 9087^T) and JJ-60^T (= CIP 111894^T = DSM 111787^T = LMG 32090^T = CCM 9086^T) as the type strains, respectively.