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Curr. Opin. Immunol. 1993 Apr;5(2):278-81
Various enzyme-linked immunosorbent assay or radioimmunoassay methods are currently used to quantify the antibody-antigen interaction. Only those based on indirect competition–enzyme-linked immunosorbent assay or radioimmunoassay–can provide the real thermodynamic affinity of the antibody for its antigen. They can be applied to a variety of experimental situations, some of which are reviewed here.