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© Research
Publication : Current opinion in immunology

Methods for measurement of antibody/antigen affinity based on ELISA and RIA

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Current opinion in immunology - 01 Apr 1993

Goldberg ME, Djavadi-Ohaniance L

Link to Pubmed [PMID] – 8507406

Curr. Opin. Immunol. 1993 Apr;5(2):278-81

Various enzyme-linked immunosorbent assay or radioimmunoassay methods are currently used to quantify the antibody-antigen interaction. Only those based on indirect competition–enzyme-linked immunosorbent assay or radioimmunoassay–can provide the real thermodynamic affinity of the antibody for its antigen. They can be applied to a variety of experimental situations, some of which are reviewed here.

https://www.ncbi.nlm.nih.gov/pubmed/8507406