Présentation
The peptidoglycan (PGN) is an essential macromolecule of bacteria composed of a glycan moiety cross-linked by small stem peptides. The assembly of this macromolecule is achieved by protein complexes that involve synthetases, scaffolding proteins and hydrolases. Most bacteria have two machineries, the elongasome being involved in building the PGN of the cell body, and the divisome building the PGN at the septum during cell division. Our objective is to combine high-through put genetics, transcriptomics and proteomics approaches to identify the entire repertoire of proteins involved in these proteins complexes. For this purpose, we use Helicobacter pylori as a model organism to study the assembly of these complexes and develop novel inhibitory compounds that interfere with the assembly of PGN complexes and can be used alone or in combination with existing PGN targeting antibiotics such as beta-lactams.
