Daniele Campisi

Désolé, cet article est seulement disponible en EN.

Education

01/09/2017 to 01/10/2021: Ph.D. in Cellular and Molecular Biology, INSERM U1239, University of Rouen-Normandy, France

Project: Impact of autophagy in glioma chemotactic migration

09/2014 to 04/2017:         M.Sc. In Cellular and Molecular Biology (specialization Neurobiology), University of Turin, Italy

Project: GPCRs and mesenchymal phenotype in high-grade gliomas

09/2011 to 07/2014:          B.Sc. in Biological Sciences (specialization Molecular Biology), University of Turin, Italy

Professional experiences

02/11/2021 to present: Post-Doctoral Fellow, Membrane Biochemistry and Transport Unit, Institut Pasteur, France

01/09/2020 to 31/08/2021: Temporary Lecturer and Research Assistant (ATER), INSERM U1239, University of Rouen-Normandy, France. Teaching: Animal Physiology (52 h, B.Sc. 2^nd year), Introduction to Cell Biology (26 h, B.Sc. 1^st year) and Histology (28 h, B.Sc. 1^st year)

03/2017 to 07/2017: ERASMUS TRAINEESHIP Program, Post-Graduate Internship, INSERM U1239, University of Rouen-Normandy, France

Project: GPCRs and mesenchymal phenotype in high-grade gliomas

15 to 17/09/2017: Masterclass in Neuro-oncology, challenges in radiotherapy for patients with gliomas, European School of Oncology (ESO) and European Association of Neuro-Oncology (EANO), Lugano, Switzerland.

02/2016 to 07/2016: ERASMUS⁺ Program, M.Sc. Interniship for Experimental thesis. INSERM U1239, University of Rouen-Normandy, France

Project: GPCRs and mesenchymal phenotype in high-grade gliomas

11/2015 to 01/2016: B.Sc. and M.Sc. Training, Neuroscience Institut Cavalieri Ottolenghi (NICO), University of Turin, Italy

Technical and lab skills

CELL BIOLOGY

Human cell cultures, Immunofluorescence, Cell transfection, Cell migration assay (Transwells and Ibidi), Bacterial protein expression, Mammalian cell protein expression, Protein-protein interaction: Yeast Two Hybrid Assay, Co-Immunoprecipitation and Pull-Down, Biomolecular Fluorescence Complementation (BiFC) with split fluorescent proteins, Prossimity assay: BioID/TurboID.

MOLECULAR BIOLOGY

Cloning and Development of recombinant plasmids, Mutagenesis, Plasmid preparation, Protein extraction/purification, SDS-PAGE, Western Blot, DNA and RNA extraction/purification, Primers design, RT-qPCR, RNA interference (siRNAs).

LIGHT MICROSCOPY AND IMAGING

Confocal microscopy, Timelapse microscopy, TIRF microscopy, Spinning Disk microscopy, Super-resolution Structured-Illumination Microscopy (SIM)

ELECTRON MICROSCOPY

Conventional EM and rOTO sample preparation for Correlative Light Electron Microscopy (CLEM). Processing Tokuyasu cryosections, Immuno-labelling for CLEM and Immuno-NanoGold for Electron Microscopy, acquisition of mosaics and tilt series with SerialEM. Tomograms and 3D reconstruction with IMOD. Microscopes: FEI Tecnai T12 cryo and T12 tomo (120 kV) and FEI Tecnai F20 (200 kV).

INFORMATIC

ImageJ, Fiji,  iMOD, SerialEM, QuantStudio (Real-time PCR data analysis), BLAST, Primer-Blast, Primer Bank, ImageLab, GraphPad Prism, Adobe Premiere, Photoshop and Illustrator, Microsoft Office (Word, Excel and PowerPoint), Leica Las X.

Membership

• 2023 – to present: Member of The French Society for Cell Biology (SBCF).
• 2022 – to present: Member of Réseau d’Imagerie en Microscopie Electronique (RIME)

Institutional activities

09/2023 to present: Post-Doc Representatives, Cell Biology and Infection Department, Institut Pasteur, France

10/2022 to present: Part of UBI User Committee, Ultrastructural BioImaging (UBI) Platform, Institut Pasteur, France

03/2012 to 09/2016: Student Representatives in the Biological Sciences Bachelor Program, Cellular and Molecular Biology Master Program and Department of Life Sciences and System Biology. University of Turin, Italy