Présentation
SINGLE CELL PROTEOMICS TO SINGLE CELL SECRETOMICS
Droplet-based microfluidics allow the reliable production of micrometer-sized incubators in which individual cells can be isolated and manipulated at high
throughput rates. Such microfluidics-based technologies have been successfully interfaced with RNA-Seq enabling the analysis of cell heterogeneity at the
transcriptome level. However, those technologies still suffer from serious limitations linked to the lack of efficient method for in-droplet biomarker capture and purification preventing the possibility to perform multi-omic investigations (transcriptomic/proteomic).
MicroOmiX addresses these limitations by combining two proprietary technologies:
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- a highly efficient and robust capture system based on functional surfactant
allowing the scavenging of a wide range of biomarkers; - a droplet washing technology based on an inversion process enabling the
separation of captured biomarkers from contaminants and other cell remnants
- a highly efficient and robust capture system based on functional surfactant
The detection of purified biomarkers can then be performed using current analyzer:
- a cytometer for a targeted approach (up to 30 RNA/protein biomarkers) or
- a sequencer for a non-targeted transcriptomic strategy combined with targeted proteomic.
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Localisation
Bâtiment: François Jacob
Adresse: Institut Pasteur, Rue du Docteur Roux, Paris, France