Lien vers Pubmed [PMID] – 40359096
Lien DOI – 10.3201/eid3113.241950
Emerg Infect Dis 2025 May; 31(13): 117-128
Whole-genome sequencing (WGS) is routine for surveillance of Shiga toxin-producing Escherichia coli human isolates in France. Protocols use EnteroBase hierarchical clustering at <5 allelic differences (HC5) as screening for cluster detection. We assessed current implementation after 5 years for 1,002 sequenced isolates. From genomic distances of serotypes O26:H11, O157:H7, O80:H2, and O103:H2, we determined statistical thresholds for cluster determination and compared those with HC5 clusters. Thresholds varied by serotype, 5-16 allelic distances and 15-20 single-nucleotide polymorphisms, showing limits of a single-threshold approach. We confirmed validity of HC5 screening for 3 serotypes because statistical thresholds had limited effect on isolate clustering (high sensitivity and specificity). For O80:H2, results suggest that HC5 is less reliable, and other approaches should be explored. Public health officials should regularly assess WGS used for Shiga toxin-producing E. coli surveillance to account for serotype and genomic evolution and to interpret WGS-linked isolates in light of epidemiologic data.