Timeline

Niels Volkmann

Head of Structure

20 Dec 2022

publication

Morphological control enables nanometer-scale dissection of cell-cell signaling complexes.

01 Dec 2022

publication

MEPSi: A tool for simulating tomograms of membrane-embedded proteins.

01 Nov 2022

publication

Characterization of heterogeneity in nanodisc samples using Feret signatures.

26 Oct 2022

publication

Novel cryo-tomography workflow reveals nanometer-scale responses of epithelial cells to matrix stiffness and dimensionality.

01 Sep 2022

publication

ECM dimensionality tunes actin tension to modulate endoplasmic reticulum function and spheroid phenotypes of mammary epithelial cells.

01 Feb 2022

publication

The complexin C-terminal amphipathic helix stabilizes the fusion pore open state by sculpting membranes.

01 Dec 2021

publication

Rapid tool for cell nanoarchitecture integrity assessment.

25 Mar 2021

publication

The actomyosin interface contains an evolutionary conserved core and an ancillary interface involved in specificity.

11 Sep 2020

publication

Structural basis of αE-catenin-F-actin catch bond behavior.

21 May 2020

publication

Factor VIII exhibits chaperone-dependent and glucose-regulated reversible amyloid formation in the endoplasmic reticulum.

06 Jan 2020

team

Structural Image Analysis

01 Nov 2019

publication

Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studies.

01 Oct 2019

publication

High Rac1 activity is functionally translated into cytosolic structures with unique nanoscale cytoskeletal architecture.

04 Jun 2019

publication

Biophysical Characterization of a Nanodisc with and without BAX: An Integrative Study Using Molecular Dynamics Simulations and Cryo-EM.

01 May 2019

publication

Electron cryo-tomography of vestibular hair-cell stereocilia.

01 Dec 2018

publication

Does self-organized criticality drive leading edge protrusion?

16 Oct 2018

publication

Local Tension on Talin in Focal Adhesions Correlates with F-Actin Alignment at the Nanometer Scale.

01 Mar 2018

publication

Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models.

01 Jan 2018

publication

Marker-free method for accurate alignment between correlated light, cryo-light, and electron cryo-microscopy data using sample support features.

01 Jan 2018

publication

Conformational Equilibrium of Human Platelet Integrin Investigated by Three-Dimensional Electron Cryo-Microscopy.

13 Nov 2017

publication

Ist1 regulates ESCRT-III assembly and function during multivesicular endosome biogenesis in Caenorhabditis elegans embryos.

17 Oct 2017

publication

Tunneling Nanotubes and Gap Junctions-Their Role in Long-Range Intercellular Communication during Development, Health, and Disease Conditions.

01 Mar 2017

publication

Nano-scale actin-network characterization of fibroblast cells lacking functional Arp2/3 complex.

01 Oct 2016

publication

Structure of anthrax lethal toxin prepore complex suggests a pathway for efficient cell entry.

23 Feb 2016

publication

Three-Dimensional Structures of Full-Length, Membrane-Embedded Human α(IIb)β(3) Integrin Complexes.

05 Nov 2015

publication

Orchestration of ErbB3 signaling through heterointeractions and homointeractions.

01 Oct 2015

publication

The IRE1α/XBP1s Pathway Is Essential for the Glucose Response and Protection of β Cells.

01 Sep 2015

publication

Validation methods for low-resolution fitting of atomic structures to electron microscopy data.

01 Sep 2015

publication

Efficient Extraction of Macromolecular Complexes from Electron Tomograms Based on Reduced Representation Templates.

01 Aug 2015

publication

Accurate membrane tracing in three-dimensional reconstructions from electron cryotomography data.

01 Apr 2015

publication

Cellular chirality arising from the self-organization of the actin cytoskeleton.

01 Mar 2015

publication

A mechanism of leading-edge protrusion in the absence of Arp2/3 complex.

31 Oct 2014

publication

Cell adhesion. The minimal cadherin-catenin complex binds to actin filaments under force.

25 Oct 2014

publication

Three-dimensional reconstructions of actin filaments capped by Arp2/3 complex.

01 Oct 2014

publication

Holoenzyme structures of endothelial nitric oxide synthase – an allosteric role for calmodulin in pivoting the FMN domain for electron transfer.

01 Jan 2014

publication

The joys and perils of flexible fitting.

01 Dec 2013

publication

αE-catenin actin-binding domain alters actin filament conformation and regulates binding of nucleation and disassembly factors.

01 Nov 2013

publication

The architectural relationship of components controlling mast cell endocytosis.

01 Oct 2013

publication

Probabilistic determination of probe locations from distance data.

01 Oct 2013

publication

Structural studies on full-length talin1 reveal a compact auto-inhibited dimer: implications for talin activation.

15 Sep 2012

publication

Toxofilin upregulates the host cortical actin cytoskeleton dynamics, facilitating Toxoplasma invasion.

15 Aug 2012

publication

Electron tomographic analysis of synaptic ultrastructure.

28 May 2012

publication

The C-terminal tail domain of metavinculin, vinculin’s splice variant, severs actin filaments.

10 Feb 2012

publication

Key structural features of the actin filament Arp2/3 complex branch junction revealed by molecular simulation.

01 Feb 2012

publication

Putting structure into context: fitting of atomic models into electron microscopic and electron tomographic reconstructions.

04 Jan 2012

publication

Three-dimensional reconstructions of Arp2/3 complex with bound nucleation promoting factors.

01 Jan 2011

publication

Correlative light-electron microscopy.

01 Jun 2010

publication

Molecular basis for the dual function of Eps8 on actin dynamics: bundling and capping.

01 Jun 2010

publication

Carboxylated N-glycans on RAGE promote S100A12 binding and signaling.

01 Jan 2010

publication

Methods for segmentation and interpretation of electron tomographic reconstructions.

01 Jul 2009

publication

Confidence intervals for fitting of atomic models into low-resolution densities.

01 Jun 2008

publication

Three-dimensional architecture of hair-bundle linkages revealed by electron-microscopic tomography.

01 Jun 2008

publication

Segmentation of electron tomographic data sets using fuzzy set theory principles.

10 Mar 2008

publication

The structural basis of actin filament branching by the Arp2/3 complex.

23 Jan 2008

publication

The structure of the C-terminal actin-binding domain of talin.

07 Nov 2007

publication

The R403Q myosin mutation implicated in familial hypertrophic cardiomyopathy causes disorder at the actomyosin interface.

24 Aug 2007

publication

Evidence for an interaction between the SH3 domain and the N-terminal extension of the essential light chain in class II myosins.

10 Aug 2007

publication

Effect of calcium on calmodulin bound to the IQ motifs of myosin V.

01 May 2007

publication

Efficient automatic noise reduction of electron tomographic reconstructions based on iterative median filtering.

01 May 2007

publication

Density-based score for selecting near-native atomic models of unknown structures.

09 Mar 2007

publication

Reconstituted NALP1 inflammasome reveals two-step mechanism of caspase-1 activation.

01 Sep 2006

publication

Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions.

20 Jan 2006

publication

Three-dimensional structure of vinculin bound to actin filaments.

01 Nov 2005

publication

Mechanism of filament nucleation and branch stability revealed by the structure of the Arp2/3 complex at actin branch junctions.

02 Sep 2005

publication

The structural basis of myosin V processive movement as revealed by electron cryomicroscopy.

17 Jan 2005

publication

Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2/3 complex function.

25 Oct 2004

publication

Automatic particle selection: results of a comparative study.

25 Oct 2004

publication

An approach to automated particle picking from electron micrographs based on reduced representation templates.

22 Oct 2004

publication

Characterization of an actin-binding site within the talin FERM domain.

13 Apr 2004

publication

Direct continuities between cisternae at different levels of the Golgi complex in glucose-stimulated mouse islet beta cells.

01 Sep 2003

publication

Full-length archaeal Rad51 structure and mutants: mechanisms for RAD51 assembly and control by BRCA2.

18 Mar 2003

publication

Myosin isoforms show unique conformations in the actin-bound state.

01 Jan 2003

publication

Electron microscopy.

01 Jan 2003

publication

Docking of atomic models into reconstructions from electron microscopy.

15 Nov 2002

publication

Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd.

25 Oct 2002

publication

A novel three-dimensional variant of the watershed transform for segmentation of electron density maps.

29 Aug 2002

publication

A little guide for advanced search:

Morphological control enables nanometer-scale dissection of cell-cell signaling complexes.

MEPSi: A tool for simulating tomograms of membrane-embedded proteins.

Characterization of heterogeneity in nanodisc samples using Feret signatures.

Novel cryo-tomography workflow reveals nanometer-scale responses of epithelial cells to matrix stiffness and dimensionality.

ECM dimensionality tunes actin tension to modulate endoplasmic reticulum function and spheroid phenotypes of mammary epithelial cells.

The complexin C-terminal amphipathic helix stabilizes the fusion pore open state by sculpting membranes.

Rapid tool for cell nanoarchitecture integrity assessment.

The actomyosin interface contains an evolutionary conserved core and an ancillary interface involved in specificity.

Structural basis of αE-catenin-F-actin catch bond behavior.

Factor VIII exhibits chaperone-dependent and glucose-regulated reversible amyloid formation in the endoplasmic reticulum.

Structural Image Analysis

Extracellular matrix micropatterning technology for whole cell cryogenic electron microscopy studies.

High Rac1 activity is functionally translated into cytosolic structures with unique nanoscale cytoskeletal architecture.

Biophysical Characterization of a Nanodisc with and without BAX: An Integrative Study Using Molecular Dynamics Simulations and Cryo-EM.

Electron cryo-tomography of vestibular hair-cell stereocilia.

Does self-organized criticality drive leading edge protrusion?

Local Tension on Talin in Focal Adhesions Correlates with F-Actin Alignment at the Nanometer Scale.

Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models.

Marker-free method for accurate alignment between correlated light, cryo-light, and electron cryo-microscopy data using sample support features.

Conformational Equilibrium of Human Platelet Integrin Investigated by Three-Dimensional Electron Cryo-Microscopy.

Ist1 regulates ESCRT-III assembly and function during multivesicular endosome biogenesis in Caenorhabditis elegans embryos.

Tunneling Nanotubes and Gap Junctions-Their Role in Long-Range Intercellular Communication during Development, Health, and Disease Conditions.

Nano-scale actin-network characterization of fibroblast cells lacking functional Arp2/3 complex.

Structure of anthrax lethal toxin prepore complex suggests a pathway for efficient cell entry.

Three-Dimensional Structures of Full-Length, Membrane-Embedded Human α(IIb)β(3) Integrin Complexes.

Orchestration of ErbB3 signaling through heterointeractions and homointeractions.

The IRE1α/XBP1s Pathway Is Essential for the Glucose Response and Protection of β Cells.

Validation methods for low-resolution fitting of atomic structures to electron microscopy data.

Efficient Extraction of Macromolecular Complexes from Electron Tomograms Based on Reduced Representation Templates.

Accurate membrane tracing in three-dimensional reconstructions from electron cryotomography data.

Cellular chirality arising from the self-organization of the actin cytoskeleton.

A mechanism of leading-edge protrusion in the absence of Arp2/3 complex.

Cell adhesion. The minimal cadherin-catenin complex binds to actin filaments under force.

Three-dimensional reconstructions of actin filaments capped by Arp2/3 complex.

Holoenzyme structures of endothelial nitric oxide synthase – an allosteric role for calmodulin in pivoting the FMN domain for electron transfer.

The joys and perils of flexible fitting.

αE-catenin actin-binding domain alters actin filament conformation and regulates binding of nucleation and disassembly factors.

The architectural relationship of components controlling mast cell endocytosis.

Probabilistic determination of probe locations from distance data.

Structural studies on full-length talin1 reveal a compact auto-inhibited dimer: implications for talin activation.

Toxofilin upregulates the host cortical actin cytoskeleton dynamics, facilitating Toxoplasma invasion.

Electron tomographic analysis of synaptic ultrastructure.

The C-terminal tail domain of metavinculin, vinculin’s splice variant, severs actin filaments.

Key structural features of the actin filament Arp2/3 complex branch junction revealed by molecular simulation.

Putting structure into context: fitting of atomic models into electron microscopic and electron tomographic reconstructions.

Three-dimensional reconstructions of Arp2/3 complex with bound nucleation promoting factors.

Correlative light-electron microscopy.

Molecular basis for the dual function of Eps8 on actin dynamics: bundling and capping.

Carboxylated N-glycans on RAGE promote S100A12 binding and signaling.

Methods for segmentation and interpretation of electron tomographic reconstructions.

Confidence intervals for fitting of atomic models into low-resolution densities.

Three-dimensional architecture of hair-bundle linkages revealed by electron-microscopic tomography.

Segmentation of electron tomographic data sets using fuzzy set theory principles.

The structural basis of actin filament branching by the Arp2/3 complex.

The structure of the C-terminal actin-binding domain of talin.

The R403Q myosin mutation implicated in familial hypertrophic cardiomyopathy causes disorder at the actomyosin interface.

Evidence for an interaction between the SH3 domain and the N-terminal extension of the essential light chain in class II myosins.

Effect of calcium on calmodulin bound to the IQ motifs of myosin V.

Efficient automatic noise reduction of electron tomographic reconstructions based on iterative median filtering.

Density-based score for selecting near-native atomic models of unknown structures.

Reconstituted NALP1 inflammasome reveals two-step mechanism of caspase-1 activation.

Type IV pilus structure by cryo-electron microscopy and crystallography: implications for pilus assembly and functions.

Three-dimensional structure of vinculin bound to actin filaments.

Mechanism of filament nucleation and branch stability revealed by the structure of the Arp2/3 complex at actin branch junctions.

The structural basis of myosin V processive movement as revealed by electron cryomicroscopy.

Effects of Arp2 and Arp3 nucleotide-binding pocket mutations on Arp2/3 complex function.

Automatic particle selection: results of a comparative study.

An approach to automated particle picking from electron micrographs based on reduced representation templates.

Characterization of an actin-binding site within the talin FERM domain.

Direct continuities between cisternae at different levels of the Golgi complex in glucose-stimulated mouse islet beta cells.

Full-length archaeal Rad51 structure and mutants: mechanisms for RAD51 assembly and control by BRCA2.

Myosin isoforms show unique conformations in the actin-bound state.

Electron microscopy.

Docking of atomic models into reconstructions from electron microscopy.

Microscopic evidence for a minus-end-directed power stroke in the kinesin motor ncd.

A novel three-dimensional variant of the watershed transform for segmentation of electron density maps.

Arp2/3 plays no role in muscle contraction.