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© Research
Publication : Journal of microbiological methods

Variation in copy number of the 28S rDNA of Aspergillus fumigatus measured by droplet digital PCR and analog quantitative real-time PCR.

Reference Center: Invasive Mycoses and Antifungals
Member: Alexandre Alanio
Member: Aude Sturny-Leclere
Member: Stéphane Bretagne
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of microbiological methods - 01 Aug 2016

Alanio A, Sturny-Leclère A, Benabou M, Guigue N, Bretagne S,

Link to Pubmed [PMID] – 27316653

Link to DOI – S0167-7012(16)30144-010.1016/j.mimet.2016.06.015

J. Microbiol. Methods 2016 08; 127(): 160-163

Droplet digital PCR (ddPCR) after DNA digestion yielded a 28S rDNA copy number of 61 to 86 copies/genome when testing 10 unrelated Aspergillus fumigatus isolates, higher than with quantitative PCR. Unfortunately, ddPCR after DNA digestion did not improve the sensitivity of our PCR assay when testing serum patients with invasive aspergillosis.