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Cloning and sequencing of PYBP, a pyrimidine-rich specific single strand DNA-binding protein

An integrative vector exploiting the transposition properties of Tn1545 for insertional mutagenesis and cloning of genes from gram-positive bacteria

© Clifton E. Barry III, Ph.D., NIAID, NIH.

Colorized scanning electron micrograph of Mycobacterium tuberculosis

Publication : Zentralblatt für Bakteriologie : international journal of medical microbiology

Use of pulsed field electrophoresis of DNA restriction fragments for comparing Listeria monocytogenes strains isolated from human infections and food in Austria

Team: Integrated Mycobacterial Pathogenomics

Member: Roland Brosch

Scientific Fields

Diseases

Organisms

Applications

Technique

Published in Zentralblatt für Bakteriologie : international journal of medical microbiology - 01 Oct 1991

Brosch R, Buchrieser C, Sixl-Voigt B, Rocourt J

Link to Pubmed [PMID] – 1755928

Zentralbl. Bakteriol. 1991 Oct;275(4):557-60

Twenty-five Listeria monocytogenes strains of human and food origin were studied, using low-frequency-cleavage restriction endonucleases and pulsed field gel electrophoresis. Sixteen different restriction patterns were obtained using ApaI and SmaI. The human isolates showed profiles different from those of the food isolates, thus strongly suggesting that the two human infections selected for this study did not originate from the contaminated foodstuffs examined at the same time. This method allowed it to clearly distinguish between strains apparently similar [i.e. belonging to the same serovar and same origin (cheese samples from the same dairy plant)].

Published on: 01 Oct 1991