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© Sandrine Etienne-Manneville
Photo prise à l'avant (dans la protrusion) d'astrocytes primaires de rat en migration. Marquage par immunofluorescence montrant en rouge, p150 Glued, une protéine associée aux extrémités 'plus' des microtubules et en vert la tubuline des microtubules. La photographie montre l'accumulation de p150 Glued à l'avant des cellules en migration, où la protéine pourrait participer à l'ancrage des microtubules à la membrane plasmique. Pour essayer de corriger, les dérèglements observés lors de la migration des cellules d'astrocytes tumuraux ou gliomes on cherche à connaitre les mécanismes moléculaires fondamentaux qui controlent la polarisation et la migration cellulaires.
Publication : Molekuliarnaia genetika, mikrobiologiia i virusologiia

[The role of genes of the system of mismatched base correction in genetic recombination of Escherichia coli]

Team: Biochemistry of Macromolecular Interactions
Member: Gouzel Karimova
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Molekuliarnaia genetika, mikrobiologiia i virusologiia - 01 Oct 1985

Karimova GA, Grigor'ev PS, Rybchin VN

Link to Pubmed [PMID] – 2948117

Mol. Gen. Mikrobiol. Virusol. 1985 Oct;(10):29-34

A genetic system was elaborated to study intramolecular recombination of bacteriophages lambda and phi 80. Practically, the ideal complementation of nucleotide sequences in recombining DNA molecules is required to obtain recombinants resulting from RecA-dependent recombination in Escherichia coli cells. a hypothesis is proposed to which the correction of mismatched bases hinders recombinant formation during recombination of fully homologous DNAs. The increased yields of hybrid molecules during interaction of the same DNA in the cells with deficient genes for correction support the hypothesis as well as independent demonstration of mutation in a gene for correction according to the effect of the increased yield of recombinants. A series of Escherichia coli cells mutants with increased formation of recombinant clones has been obtained.