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© Pierre Gounon
Virus influenza purifié, agent de la grippe. Ce virus enveloppé possède un génome fragmenté : 8 segments d'ARN négatif protégés par une nucléocapside.
Publication : Virology

The E2-E166K substitution restores Chikungunya virus growth in OAS3 expressing cells by acting on viral entry

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Virology - 11 Aug 2012

Henrik Gad H, Paulous S, Belarbi E, Diancourt L, Drosten C, Kümmerer BM, Plate AE, Caro V, Desprès P

Link to Pubmed [PMID] – 22889614

Virology 2012 Dec;434(1):27-37

Human 2′,5′-oligoadenylate synthetase 3 (OAS3) exerts antiviral effect against alphaviruses including Chikungunya virus (CHIKV) by inhibiting viral RNA accumulation. Here, we identified a CHIKV variant exhibiting a remarkable resistance to the antiviral action of OAS3 in human epithelial HeLa cells. Using a molecular clone of CHIKV with Renilla luciferase inserted as a reporter gene in the non-structural region, we demonstrated that a single glutamine-to-lysine amino acid change at position 166 of the envelope E2 glycoprotein restores CHIKV replication in OAS3 expressing HeLa cells. Viral entry assays showed that CHIKV with a lysine at position E2-166 was more efficient at entering the replicative pathway. The E2-E166K substitution promotes a greater efficiency of CHIKV replication in human myoblasts leading to severe apoptosis through a more robust activation of the PKR pathway. These observations provide a new insight into the role of E2 into the pathogenicity of CHIKV in human cells.

https://www.ncbi.nlm.nih.gov/pubmed/22889614