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© Research
Publication : Methods in Molecular Biology

Systematic Determination of Transcription Factor DNA-Binding Specificities in Yeast

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in Molecular Biology - 28 Jul 2015

Peña-Castillo L and Badis G.

Methods Molecular Biology, Vol. 1361, Frédéric Devaux (Eds): Yeast Functional Genomics, 978-1-4939-3078-4, 318712_1_En, (12)

Understanding how genes are regulated, decoding their “regulome”, is one of the main challenges of the post-genomic era. Here, we describe the in vitro method we used to associate cis-regulatory sites with cognate trans-regulators by characterizing the DNA-binding specificity of the vast majority of yeast transcription factors using Protein Binding Microarrays. This approach can be implemented to any given organism.