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© Research
Publication : Applied and environmental microbiology

Simple method for repurification of endotoxins for biological use

Member: Catherine Werts
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Applied and environmental microbiology - 19 Jan 2007

Tirsoaga A, Novikov A, Adib-Conquy M, Werts C, Fitting C, Cavaillon JM, Caroff M

Link to Pubmed [PMID] – 17261511

Appl. Environ. Microbiol. 2007 Mar;73(6):1803-8

A method for obtaining highly purified endotoxin (lipopolysaccharide [LPS]) in a few hours by repurification of commercial or laboratory preparations was devised. It avoids the use of phenol, which is not suitable for phenol-soluble lipopolysaccharides nor for some industrial purposes. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis and matrix-assisted laser desorption ionization mass spectrometry analysis confirmed the integrity of the purified LPSs. The purified products did not activate Toll-like receptor 2 (TLR2), nuclear oligomerization domain 1 (NOD1), or NOD2 but did activate TLR4. Applied to different lipopolysaccharides, the method also improved their mass spectra, thus facilitating their structural analysis.