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© Research
Publication : Infection and immunity

Signature-tagged transposon mutagenesis identifies novel Mycobacterium tuberculosis genes involved in the parasitism of human macrophages

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Infection and immunity - 09 Oct 2006

Rosas-Magallanes V, Stadthagen-Gomez G, Rauzier J, Barreiro LB, Tailleux L, Boudou F, Griffin R, Nigou J, Jackson M, Gicquel B, Neyrolles O

Link to Pubmed [PMID] – 17030567

Infect. Immun. 2007 Jan;75(1):504-7

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

http://www.ncbi.nlm.nih.gov/pubmed/17030567