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© Melanie Blokesch, EPFL
Flagellated Vibrio cholerae
Publication : Methods in molecular biology (Clifton, N.J.)

Shuffling of DNA cassettes in a synthetic integron

Team: Bacterial Genome Plasticity
Team: Synthetic Biology
Member: Didier Mazel
Member: David Bikard
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Methods in molecular biology (Clifton, N.J.) - 01 Jan 2013

Bikard D, Mazel D

Link to Pubmed [PMID] – 23996447

Methods Mol. Biol. 2013;1073:169-74

The complexity of even small gene networks makes them hardly amenable to rational design. Testing random combinations of genetic elements in a directed evolution procedure is thus of interest for many applications including metabolic engineering. Here we describe how the recombination machinery of class 1 integrons can be used to deliver and shuffle genetic elements at a chromosomal locus in E. coli.