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© Therese Couderc, Marc Lecuit
Publication : Cell reports

Rif1 Binding and Control of Chromosome-Internal DNA Replication Origins Is Limited by Telomere Sequestration

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Cell reports - 24 Apr 2018

Hafner L, Lezaja A, Zhang X, Lemmens L, Shyian M, Albert B, Follonier C, Nunes JM, Lopes M, Shore D, Mattarocci S

Link to Pubmed [PMID] – 29694906

Cell Rep 2018 Apr;23(4):983-992

The Saccharomyces cerevisiae telomere-binding protein Rif1 plays an evolutionarily conserved role in control of DNA replication timing by promoting PP1-dependent dephosphorylation of replication initiation factors. However, ScRif1 binding outside of telomeres has never been detected, and it has thus been unclear whether Rif1 acts directly on the replication origins that it controls. Here, we show that, in unperturbed yeast cells, Rif1 primarily regulates late-replicating origins within 100 kb of a telomere. Using the chromatin endogenous cleavage ChEC-seq technique, we robustly detect Rif1 at late-replicating origins that we show are targets of its inhibitory action. Interestingly, abrogation of Rif1 telomere association by mutation of its Rap1-binding module increases Rif1 binding and origin inhibition elsewhere in the genome. Our results indicate that Rif1 inhibits replication initiation by interacting directly with origins and suggest that Rap1-dependent sequestration of Rif1 increases its effective concentration near telomeres, while limiting its action at chromosome-internal sites.

https://www.ncbi.nlm.nih.gov/pubmed/29694906