Search anything and hit enter
  • Teams
  • Members
  • Projects
  • Events
  • Calls
  • Jobs
  • publications
  • Software
  • Tools
  • Network
  • Equipment

A little guide for advanced search:

  • Tip 1. You can use quotes "" to search for an exact expression.
    Example: "cell division"
  • Tip 2. You can use + symbol to restrict results containing all words.
    Example: +cell +stem
  • Tip 3. You can use + and - symbols to force inclusion or exclusion of specific words.
    Example: +cell -stem
e.g. searching for members in projects tagged cancer
Search for
Count
IN
OUT
Content 1
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Content 2
  • member
  • team
  • department
  • center
  • program_project
  • nrc
  • whocc
  • project
  • software
  • tool
  • patent
  • Administrative Staff
  • Assistant Professor
  • Associate Professor
  • Clinical Research Assistant
  • Full Professor
  • Graduate Student
  • Lab assistant
  • Non-permanent Researcher
  • Permanent Researcher
  • Pharmacist
  • PhD Student
  • Physician
  • Post-doc
  • Project Manager
  • Research Associate
  • Research Engineer
  • Retired scientist
  • Technician
  • Undergraduate Student
  • Veterinary
  • Visiting Scientist
  • Deputy Director of Center
  • Deputy Director of Department
  • Deputy Director of National Reference Center
  • Deputy Head of Facility
  • Director of Center
  • Director of Department
  • Director of Institute
  • Director of National Reference Center
  • Group Leader
  • Head of Facility
  • Head of Operations
  • Head of Structure
  • Honorary President of the Departement
  • Labex Coordinator
Search
Go back
Scroll to top
Share
© Research
Publication : The EMBO journal

Reverse transcriptases and genomic variability: the accuracy of DNA replication is enzyme specific and sequence dependent

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in The EMBO journal - 01 May 1990

Ricchetti M, Buc H

Link to Pubmed [PMID] – 1691709

EMBO J. 1990 May;9(5):1583-93

Kinetics of incorporation of correct and incorrect deoxynucleotides by three reverse transcriptases have been followed, by gel assay, on a series of DNA templates, including part of the HIV-1 gag DNA minus strand. Insertion kinetics for the properly matched nucleotide at a given place on the template vary strongly from one enzyme to the next. No significant correlation is found between the site-specific Michaelis constants, while the maximal velocities are more closely connected. For a given reverse transcriptase these parameters are strongly influenced by the DNA sequence. A systematic evaluation of the frequencies of misincorporation was then performed at 46 positions. Again great variability was found, precluding a very accurate evaluation of an average misincorporation frequency for a given enzyme and a given mismatch. Qualitatively however, HIV-1 reverse transcriptase is certainly not more error-prone in this assay than the other enzymes assayed. The patterns of misincorporations were again very dependent on the enzyme used to replicate a given template. The variability of the gag sequence observed in vivo among various HIV-1 isolates was compared with the patterns of misincorporations obtained in vitro on the same sequence with HIV-1, AMV and MoMLV reverse transcriptases. A fair agreement was found with the pattern observed in the polymerization directed by the HIV-1 reverse transcriptase. The correlation is less important in the two other cases. However some specific changes observed in vivo cannot be accounted for by our misincorporation assay, even when performed with the homologous enzyme, suggesting that an important class of mismatches can only be generated during reverse transcription of the RNA strand. Additional data, using a complementary DNA (positive) strand as a gag template support this hypothesis.

http://www.ncbi.nlm.nih.gov/pubmed/1691709