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© Perrine Bomme, Guillaume Duménil, Jean-Marc Panaud.
Coloured scanning electron micrograph (SEM) of Neisseria meningitidis on epithelial cells
Publication : FEMS microbiology reviews

Quality assessed nonculture techniques for detection and typing of meningococci.

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in FEMS microbiology reviews - 01 Jan 2007

Taha MK, Fox A,

Link to Pubmed [PMID] – 17233634

FEMS Microbiol Rev 2007 Jan; 31(1): 37-42

PCR protocols are increasingly used in laboratories worldwide for the diagnosis and confirmation of invasive meningococcal infection. Protocols are now available for the identification of Neisseria meningitidis, for genogrouping, susceptibility to antibiotics and genotyping of the corresponding isolates. The implementation of quality assurance (QA) schemes and standardization of protocols are required. Diagnostic and confirmatory PCRs should perform consistently in clinical and reference microbiology laboratories. General QA schemes address the issues of sample preparation, PCR laboratory environment, equipment and validation of protocols. Moreover, external QA interlaboratory studies are essential. The European Monitoring Group on Meningococci has provided a good forum to conduct such studies through the development and distribution of samples and protocols for nonculture detection and typing of N. meningitidis.

https://pubmed.ncbi.nlm.nih.gov/17233634