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© Research
Publication : Endocrinology

Pulsatile exocytosis is functionally associated with GnRH gene expression in immortalized GnRH-expressing cells

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Endocrinology - 01 Dec 2001

Vazquez-Martinez R, Shorte SL, Faught WJ, Leaumont DC, Frawley LS, Boockfor FR

Link to Pubmed [PMID] – 11713236

Endocrinology 2001 Dec;142(12):5364-70

Pulsatile release of GnRH is essential for proper reproductive function, but little information is available on the molecular processes underlying this intermittent activity. Recently, GnRH gene expression (GnRH-GE) episodes and exocytotic pulses have been identified separately in individual GnRH-expressing cells, raising the exciting possibility that both activities are linked functionally and are fundamental to the pulsatile process. To explore this, we monitored GnRH-GE (using a GnRH promoter-driven luciferase reporter) and exocytosis (by FM1-43 fluorescence) in the same, living GT1-7 cells. Our results revealed a strong temporal association between exocytotic pulses and GnRH-GE episodes. To determine whether a functional link existed, we blocked one process and evaluated the other. Transcriptional inhibition with actinomycin D had only a modest influence on exocytosis, suggesting that exocytotic pulse activity was not dictated acutely by episodes of gene expression. In contrast, blockage of exocytosis with anti-SNAP-25 (which obstructs secretory granule fusion) abolished GnRH-GE pulse activity, indicating that part of the exocytotic process is responsible for triggering episodes of GnRH-GE. When taken together, our findings suggest that a careful balance is maintained between release and biosynthesis in GT1-7 cells. Such a property may be important in the hypothalamus to ensure that GnRH neurons are in a constant state of readiness to respond to changes in reproductive function.

https://www.ncbi.nlm.nih.gov/pubmed/11713236