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© Christelle Durand
Microscopie d'un neurone. Le marquage jaune montre les synapses.
Publication : Protein expression and purification

Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Protein expression and purification - 03 Aug 2010

Ben-Abdallah M, Bondet V, Fauchereau F, Béguin P, Goubran-Botros H, Pagan C, Bourgeron T, Bellalou J

Link to Pubmed [PMID] – 20688166

Protein Expr. Purif. 2011 Jan;75(1):114-8

N-acetyl serotonin methyl transferase (ASMT) is the last enzyme in the melatonin synthesis pathway. Evidence linking autism-related disorders with disorders of melatonin metabolism, and, more specifically, with mutations of the gene encoding ASMT, prompted us to investigate the properties and localization of this enzyme. As a first step, we undertook to overproduce the protein in a recombinant host. Early attempts to produce ASMT in recombinant Escherichia coli yielded only insoluble and heavily degraded material. However, recombinant ASMT (rASMT) could be produced in soluble, active form and purified in milligram amounts when the gene was cloned and expressed in Leishmania tarentolae.

http://www.ncbi.nlm.nih.gov/pubmed/20688166