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© Christine Schmitt, Anubis Vega Rua, Jean-Marc Panaud
Tête de moustique femelle Aedes albopictus, vecteur du virus de la dengue et du chikungunya. Microphotographie électronique à balayage, image colorisée.
Publication : Annales de l'Institut Pasteur. Virology

Preparation of high-titred antisera for sigma virus strains from Hawaii and France and their use in neutralization tests in vitro and in vivo

Team: Arboviruses and Insect Vectors
Member: Marie Vazeille
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Annales de l'Institut Pasteur. Virology - 01 Apr 1988

Vazeille MC, Rosen L

Link to Pubmed [PMID] – 3144993

Ann. Inst. Pasteur Virol. 1988 Apr-Jun;139(2):175-83

Antisera were prepared in rabbits against two strains of sigma virus, from France and Hawaii respectively, using purified virus from cell cultures. All antisera had the same titre by immunofluorescence, 1/400 or 1/800, whether tested against the homologous or the heterologous virus. An antiserum prepared with the French strain neutralized the Hawaiian virus at a dilution of 1/800 both in vitro and in vivo. The Hawaiian virus was not neutralized to a significant extent by reference mouse ascitic fluids for 26 different vertebrate rhabdoviruses.