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© Research
Publication : Current opinion in immunology

Polymerase chain reaction for detection of cytokine gene expression

Team: Group: Humoral Immunity
Member: Paulo Vieira
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Current opinion in immunology - 01 Apr 1992

O'Garra A, Vieira P

Link to Pubmed [PMID] – 1376605

Curr. Opin. Immunol. 1992 Apr;4(2):211-5

A powerful method to amplify reverse-transcribed RNA, the polymerase chain reaction can be used to measure cytokine gene transcription in a small number of cells, or in cases where there is low mRNA copy number. This technique may be used to obtain qualitative or quantitative determinations of cytokine gene expression. In this review we discuss the various strategies recently described for the evaluation of cytokine expression using the polymerase chain reaction.