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© Artur Scherf
Scanning Electron Microscopy of Red Blood Cell infected by Plasmodium falciparum.
Publication : Journal of immunological methods

Plaque antibody selection: rapid immunological analysis of a large number of recombinant phage clones positive to sera raised against Plasmodium falciparum antigens

Scientific Fields
Diseases
Organisms
Applications
Technique

Published in Journal of immunological methods - 22 May 1986

Ozaki LS, Mattei D, Jendoubi M, Druihle P, Blisnick T, Guillotte M, Puijalon O, Da Silva LP

Link to Pubmed [PMID] – 3517177

J. Immunol. Methods 1986 May;89(2):213-9

A library of Plasmodium falciparum genomic DNA on the lambda gt11 phage vector was screened for clones positive to a rabbit serum raised against a purified fraction of P. falciparum proteins and a pool of sera from malaria patients. The positive clones were characterized with antibodies purified by the plaque antibody selection technique. This technique consist of purifying specific antibodies on a nitrocellulose filter blotted directly on a lawn of plaques of an antigen-producing phage clone. The purified antibodies are then used as a probe in a Western blot of parasite protein extract, for preliminary characterization of the clones. Using this method, two different clones coding for P. falciparum antigens were identified with the rabbit serum and about 20 with the human sera. This method can be of general use, i.e. it is not limited to parasite systems, and facilitates the immunological analysis and identification of a large number of clones.

http://www.ncbi.nlm.nih.gov/pubmed/3517177