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© A. Chauhan, JM Ghigo, C. Beloin.
Biofilm formation by Pseudomonas aeruginosa bacteria in Central Venous Catheter. Bacteria are stained in yellow and blood cells in red.
Publication : FEMS Microbiology Letters

Overproduction, purification and characterization of the HPB12-L24 ribosomal protein of Bacillus subtilis.

Team: Group: Christophe Beloin
Member: Christophe Beloin
Scientific Fields
Diseases
Organisms
Applications
Technique

Published in FEMS Microbiology Letters - 15 Nov 1996

Zouine M, Beloin C, Deneubourg AM, Hirschbein L, Le Hegarat F.

Link to Pubmed [PMID] – 8931325

FEMS Microbiol Lett. 1996 Nov 15;145(1):41-8.

HPB12-L24 was previously described as a bifunctional histone-like and ribosomal protein in Bacillus subtilis. In order to confirm the identity of HPB12 and L24, and to study the properties of this protein, the rplX gene of B. subtilis encoding L24 has been overexpressed in Escherichia coli by an efficient protein overproduction system. A simple and rapid purification scheme using ammonium sulfate precipitation and cation-exchange chromatography is presented. 10 mg of pure L24 per g of Escherichia coli cells were obtained. The purified recombinant protein L24 is heat-stable, acid-soluble and binds preferentially supercoiled DNA like protein HPB12. These results confirm the identity of HPB12 and L24. Overexpression of rplX led to gross alterations of cell morphology and to an abnormal shape of nucleoids.